Natural Transformation in Vibrio parahaemolyticus: a Rapid Method To Create Genetic Deletions

Spreading of vibrios throughout the world correlates with increased global temperatures. As they spread, they find new niches in which to survive, proliferate, and invade. Therefore, genetic manipulation of vibrios is of the utmost importance for studying these species. Here, we have delineated and validated a rapid method to create genetic deletions in Vibrio parahaemolyticus . This study provides insightful methodology for studies with other Vibrio species.