Phenotypic characterisation and detection of antibiotic resistance genes of Truepe¬rella pyogenes associated with bovine endometritis

Endometritis is considered an essential factor affecting both the longevity and profitability of a dairy herd. It causes enormous economic losses and is usually categorised as clinical and subclinical endometritis. Trueperella pyogenes can cause numerous infections in the organs and tissues of different farm animals. T. pyogenes is a clinically significant bacterium that causes severe endometritis, particularly in postpartum dairy cows. A total of 110 uterine swab samples from cows (75 cows with subclinical endometritis and 35 cows with clinical endometritis) were used in different veterinary clinics at Kaliobia Governorate, Egypt, for isolation of T. pyogenes strains, phenotypic identification and genotypic characterisation of antibiotic resistance genes. T. pyogenes was recovered from 30 examined uterine samples (27.3%). The isolates displayed greater resistance to tetracycline than to oxacillin, ampicillin, streptomycin, co-trimoxazole and doxycycline; nevertheless, they were sensitive to gentamicin, cephapirin, ciprofloxacin, norfloxacin, amoxicillin/clavulanic acid and cefotaxime. PCR showed that all five studied T. pyogenes strains had the bla gene and four strains carried aadA1 & tet(W) genes. T. pyogenes was resistant to multiple antimicrobial drugs and had antibiotic resistance genes; hence, it is a significant bacterium causing bovine endometritis.