Association of Adenovirus with the MicrotubuleOrganizingCenter
Key result
In enucleated cells, the majority of Adenovirus capsids established a stable interaction with the microtubule organizing center, suggesting nuclear factors are required for dissociation.
Population
Enucleated A549 human lung epithelial cells
Comparison
Infection with Cy3-labeled Adenovirus vs Intact, mock-enucleated A549 cells
Design
Preclinical
Follow-up
more than 5 h after infection
Authors
Discussion
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Member takes
Implication
Hypothesis-generating for nuclear roles in adenovirus trafficking; requires confirmation in nucleated cells before extending to vector design.
Structured PICO
P
Population
Enucleated A549 human lung epithelial cells
I
Intervention
Infection with Cy3-labeled Adenovirus
C
Comparator
Intact, mock-enucleated A549 cells
O
Outcome
Adenovirus capsid trafficking and localization to the microtubule organizing center (MTOC)
Adenovirus capsids establish a stable interaction with the MTOC in the absence of a nucleus, suggesting nuclear factors are required for dissociation from microtubules.
Cite This Study
Bailey et al. (2003) studied Adenovirus infection (in vitro). Enucleation of cells vs. Intact, mock-enucleated cells was evaluated on Adenovirus capsid trafficking and localization to the MTOC. In enucleated cells, the majority of Adenovirus capsids established a stable interaction with the microtubule organizing center, suggesting nuclear factors are required for dissociation.