Key result
Dimethyl sulfate footprinting and LM-PCR revealed that adenovirus type 5 contains sequence- or locus-specific DNA binding proteins adjacent to both inverted terminal repeats within the virion.
Adenovirus type 5 contains sequence- or locus-specific DNA binding proteins adjacent to inverted terminal repeats within the virion.
Adenoviral DNA was examined within capsids by dimethyl sulfate footprinting. Protein-DNA interactions were visualized through ligation-mediated PCR (LM-PCR). Signals for protein binding were found adjacent to both inverted terminal repeats (ITR). There were no indications of close protein binding at several other loci of the viral genome. Therefore, adenovirus type 5 seems to contain sequence- or locus-specific DNA binding proteins within the virion.
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Dobner et al. (2001) studied this question. Dimethyl sulfate footprinting and LM-PCR revealed that adenovirus type 5 contains sequence- or locus-specific DNA binding proteins adjacent to both inverted terminal repeats within the virion.
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