Key result
hhLIM protein promoted the specific expression of the ANF gene in H9c2 cardiomyoblast cells by interacting and co-operating with the cardiac-restricted transcription factor Nkx2.5.
hhLIM acts as a cofactor with the cardiac-restricted transcription factor Nkx2.5 to promote specific expression of the ANF gene, providing insight into the molecular mechanisms of cardiac hypertrophy.
hhLIM [human heart LIM (Lin-11/IsI-1/Mec-3) protein] is a muscle-specific LIM-only protein that consists of two LIM motifs. hhLIM functions as a positive regulator for cardiac hypertrophy. Here we report that hhLIM serves as a cofactor regulating the expression of the ANF (atrial natriuretic factor) gene in H9c2 rat cardiomyoblast cells. We found that hhLIM promoted the expression of the ANF gene in H9c2 cells, but not in A293 human embryonic kidney cells. Furthermore, we showed that hhLIM interacted with Nkx2.5 (a cardiac-restricted transcription factor) in vivo and in vitro using its N-terminal LIM domain and enhanced the binding ability of Nkx2.5 to the NKE (Nkx2.5-binding element) boxes in the ANF promoter. These results suggest that hhLIM promotes the specific expression of the ANF gene by co-operating with Nkx2.5.
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Zheng et al. (2008) studied this question. hhLIM protein was evaluated on ANF gene expression and interaction with Nkx2.5. hhLIM protein promoted the specific expression of the ANF gene in H9c2 cardiomyoblast cells by interacting and co-operating with the cardiac-restricted transcription factor Nkx2.5.
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