April 1, 1991Molecular and Cellular Biology

A Conserved 28-Base-Pair Element (HF-1) in the Rat Cardiac Myosin Light-Chain-2 Gene Confers Cardiac-Specific and α-Adrenergic-Inducible Expression in Cultured Neonatal Rat Myocardial Cells

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Population

Cultured neonatal rat myocardial cells, mouse myocardium, Sol8 and C2/C12 mouse skeletal muscle cells, and…

Comparison

Transfection with wild-type and mutant… vs Wild-type MLC-2 promoter constructs…

Design

Preclinical

Authors

HZHong ZhuUniversity of Science and Technology of China AGArnold GarciaUniversity of California, San Diego RRRobert S. RossPurbanchal University

Discussion

Member takes

Implication

Identifies HF-1/HF-2 as key for cardiac gene control in hypertrophy; leaves open in vivo and human translation.

Structured PICO

Population

Cultured neonatal rat myocardial cells (from 1- to 2-day-old Sprague-Dawley rats), mouse myocardium, Sol8 and C2/C12 mouse skeletal muscle cells, and CV-1 nonmuscle cells.

Intervention

Transfection with wild-type and mutant MLC-2-luciferase constructs (mutations in HF-1, HF-2, HF-3 regions) and stimulation with phenylephrine or endothelin-1.

Comparator

Wild-type MLC-2 promoter constructs, unstimulated cells, and non-cardiac cell lines.

Outcome

Luciferase reporter activity (transcriptional expression level).surrogate

The HF-1 regulatory element is both necessary and sufficient for cardiac-specific and inducible expression of the MLC-2 gene during myocardial cell hypertrophy.

Cite This Study

Zhu et al. (1991) studied this question.

synapsesocial.com/papers/6a751537756843cacbb1e69a https://doi.org/10.1128/mcb.11.4.2273-2281.1991

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A Conserved 28-Base-Pair Element (HF-1) in the Rat Cardiac Myosin Light-Chain-2 Gene Confers Cardiac-Specific and α-Adrenergic-Inducible Expression in Cultured Neonatal Rat Myocardial Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

A Conserved 28-Base-Pair Element (HF-1) in the Rat Cardiac Myosin Light-Chain-2 Gene Confers Cardiac-Specific and α-Adrenergic-Inducible Expression in Cultured Neonatal Rat Myocardial Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider