Key result
A combination of positive (HF-1a/HF-1b) and negative (E-box and HF-3) regulatory elements is required to maintain ventricular chamber-specific expression of the MLC-2v gene in transgenic mice.
Population
50 independent transgenic founder mice harboring mutations in putative MLC-2 cis regulatory elements in a…
Comparison
Mutations in HF-1a, HF-1b, HF-2, HF-3, and E-box… vs Wild-type 250-bp MLC-2-luciferase fusion gene
Design
Preclinical
Authors
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Animal data on MLC-2v regulation should not yet guide clinical gene targeting; leaves open validation in human cardiomyocytes.
Ventricular chamber-specific expression of the MLC-2v gene in vivo requires a combination of positive (HF-1a/HF-1b) and negative (E-box and HF-3) regulatory elements.
Lee et al. (1994) studied Ventricular muscle-specific gene expression. Mutations in putative MLC-2 cis regulatory elements (HF-1, HF-2, HF-3, E-box) vs. Unmutated regulatory elements was evaluated on Luciferase reporter activity (ventricular chamber-specific expression). A combination of positive (HF-1a/HF-1b) and negative (E-box and HF-3) regulatory elements is required to maintain ventricular chamber-specific expression of the MLC-2v gene in transgenic mice.
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