What is the clinical evidence from this study?

Study design: Other. Population: Cardiac ventricular gene expression. Intervention: HF-1a/HF-1b/MEF-2 combinatorial element. Primary outcome: Ventricular-specific reporter expression.

What question did this study set out to answer?

This research aims to identify the molecular determinants for ventricular-specific gene expression and the anterior/posterior axis in the developing heart.

June 1, 1996Development

An HF-1a/ HF-1b/MEF-2 combinatorial element confers cardiac ventricular specificity and establishes an anterior-posterior gradient of expression

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Key result

A 250-bp myosin light chain-2 ventricular promoter fragment and a 28-bp HF-1 sub-element directed right ventricular/conotruncal dominant reporter expression independently of RXR-alpha and Nkx2-5.

Population

Transgenic mouse lines, including retinoid X receptoralpha and Nkx2-5 null backgrounds

Design

Preclinical

Authors

RRRobert S. RossPurbanchal University SNSutip NavankasattusasUniversity of Utah RHRichard P. HarveyNavicent Health

Discussion

Member takes

Implication

Offers a minimal model for ventricular patterning; leaves open its relevance to human heart development.

Key Points

This research aims to identify the molecular determinants for ventricular-specific gene expression and the anterior/posterior axis in the developing heart.
Utilized myosin light chain-2 ventricular promoter/beta-galactosidase reporter transgenes in multiple transgenic mouse lines.
Analyzed transgene expression across different mouse strains and in various mutant backgrounds including retinoid X receptoralpha and Nkx2-5 nulls.
Assessed the temporospatial characteristics of the transgene expression during heart development.
The HF-1 element directs ventricular-specific reporter expression as early as day 7.5-8.0 post coitum.
Transgene expression was observed in both mutant backgrounds, indicating independence from retinoid X receptoralpha and Nkx2-5.
The study suggests a minimal combinatorial pathway for ventricular gene expression.

Structured PICO

Population

Transgenic mouse lines (myosin light chain-2 ventricular promoter/beta-galactosidase reporter transgenes), including retinoid X receptoralpha and Nkx2-5 null backgrounds

Intervention

250 base pair myosin light chain-2 ventricular promoter fragment and a dimerized 28 bp sub-element (HF-1)

Outcome

Ventricular-specific reporter expressionsurrogate

The HF-1 element defines a minimal combinatorial pathway for ventricular chamber gene expression, providing a model to study anterior-posterior gradients during mammalian heart development.

Cite This Study

Ross et al. (1996) studied Cardiac ventricular gene expression. HF-1a/HF-1b/MEF-2 combinatorial element was evaluated on Ventricular-specific reporter expression. A 250-bp myosin light chain-2 ventricular promoter fragment and a 28-bp HF-1 sub-element directed right ventricular/conotruncal dominant reporter expression independently of RXR-alpha and Nkx2-5.

synapsesocial.com/papers/6a0d51f2cae7912d2fa4e734 https://doi.org/10.1242/dev.122.6.1799

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An HF-1a/ HF-1b/MEF-2 combinatorial element confers cardiac ventricular specificity and establishes an anterior-posterior gradient of expression

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

An HF-1a/ HF-1b/MEF-2 combinatorial element confers cardiac ventricular specificity and establishes an anterior-posterior gradient of expression

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

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