Key result
Deletion of the D3 region from RyR1 and residues 1038-3355 from RyR2 rendered the channels resistant to inactivation by mM Ca2+, suggesting the skeletal D3 region is involved in Ca2+ regulation.
Population
Skeletal (RyR1) and cardiac (RyR2) muscle cell ryanodine receptor/Ca2+ release channels
Design
Preclinical
Authors
Loading...
D3 region may modulate RyR1 Ca2+ sensitivity; leaves open validation in native muscle before therapeutic consideration.
The skeletal D3 region of the ryanodine receptor is involved in the Ca2+-dependent regulation and inactivation of the RyR1 channel.
Hayek et al. (1999) studied this question. Deletion or insertion of the D3 region in RyR1 and RyR2 channels vs. Wild-type or other chimeric channels was evaluated on Channel inactivation by cytosolic Ca2+ and Mg2+. Deletion of the D3 region from RyR1 and residues 1038-3355 from RyR2 rendered the channels resistant to inactivation by mM Ca2+, suggesting the skeletal D3 region is involved in Ca2+ regulation.
Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context: