Key result
Mutation of glutamates E702 and E705 altered Ca2+ sensitivity, indicating that the putative fourth extracellular loop of Ano1 is intracellular and may contain a Ca2+ binding site.
The study proposes a revised topology for Ano1 chloride channels, showing that the putative fourth extracellular loop is intracellular and may contain a Ca2+ binding site.
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Revises Ano1 topology models; leaves open Ca2+ site as therapeutic target pending mammalian validation.
Yu et al. (2012) studied this question. Mutation of glutamates E702 and E705 in Ano1 was evaluated on Ca2+ sensitivity of Ano1. Mutation of glutamates E702 and E705 altered Ca2+ sensitivity, indicating that the putative fourth extracellular loop of Ano1 is intracellular and may contain a Ca2+ binding site.
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