What question did this study set out to answer?

To identify human βV-tubulin protein expression in epithelial cancer cell lines and compare its characteristics and expression pattern to other β-tubulin isotypes.

November 8, 2005Biochemistry

Detection of Human βV-Tubulin Expression in Epithelial Cancer Cell Lines by Tubulin Proteomics

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Authors

PVPascal Verdier‐PinardCentre National de la Recherche Scientifique SSShohreh ShahabiNorthwestern University FWFang WangSouth China Agricultural University

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Implication

Proteomic analysis reveals high expression of human betaV-tubulin in ovarian cancer cells, suggesting a complementary role with betaIII-tubulin in modulating cellular sensitivity to Taxol.

Key Points

To identify human βV-tubulin protein expression in epithelial cancer cell lines and compare its characteristics and expression pattern to other β-tubulin isotypes.
Analyzed tubulin isotypes in human epithelial cancer cell lines, including the Hey ovarian cancer cell line.
Combined high-resolution isoelectric focusing, in-gel cyanogen bromide (CNBr) cleavage, and mass spectrometry for protein detection and characterization.
Detected human βV-tubulin protein expression for the first time in human cell lines, with high expression observed in the Hey epithelial ovarian cancer line.
Confirmed distinct structural sequence differences between human and rodent βV-tubulin at their respective C-termini.
Identified a complementary protein expression pattern between βIII-tubulin and βV-tubulin across species.

Cite This Study

Verdier‐Pinard et al. (2005) studied this question.

synapsesocial.com/papers/6a969ecbda4ae31144f6a6dc https://doi.org/10.1021/bi051004p

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Implication

Proteomic analysis reveals high expression of human betaV-tubulin in ovarian cancer cells, suggesting a complementary role with betaIII-tubulin in modulating cellular sensitivity to Taxol.

Detection of Human βV-Tubulin Expression in Epithelial Cancer Cell Lines by Tubulin Proteomics

Discussion

Member takes

Implication

Key Points

Cite This Study

Detection of Human βV-Tubulin Expression in Epithelial Cancer Cell Lines by Tubulin Proteomics

Discussion

Member takes

Implication

Key Points

Cite This Study

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