What question did this study set out to answer?

To determine whether urocortin prevents cardiac myocyte death and reduces myocardial injury during ischemia and reperfusion, and to identify the underlying MAPK-dependent signaling mechanisms.

March 1, 2000Journal of Biological ChemistryOpen Access

Urocortin Protects against Ischemic and Reperfusion Injury via a MAPK-dependent Pathway

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Authors

BBBhawanjit K. BrarSalk Institute for Biological Studies AJAnna K. JonassenUniversity College Hospital ASAnastasis StephanouInstitut polytechnique de Grenoble

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Implication

Experimental study demonstrates that urocortin reduces cardiac damage in rat heart models, suggesting a therapeutic role during reperfusion after ischemic events.

Key Points

To determine whether urocortin prevents cardiac myocyte death and reduces myocardial injury during ischemia and reperfusion, and to identify the underlying MAPK-dependent signaling mechanisms.
Administered urocortin to primary rat cardiac myocyte cultures before simulated hypoxia/ischemia and at reoxygenation, measuring cell survival via trypan blue exclusion, TUNEL, annexin V, and flow cytometry.
Involved the MEK1-ERK1/2 inhibitor PD98059 to evaluate the role of ERK1/2-p42/44 phosphorylation in urocortin-mediated cardioprotection.
Tested urocortin administration before ischemia or at reperfusion in isolated rat hearts subjected to regional ischemia/reperfusion ex vivo.
Urocortin significantly prevented cardiac myocyte death when administered either prior to simulated ischemia or at the onset of reoxygenation.
Urocortin stimulated rapid phosphorylation of ERK1/2-p42/44, and pharmacological blockade of the MEK1-ERK1/2 pathway with PD98059 completely inhibited its survival-promoting effect.
Ex vivo urocortin administration markedly reduced myocardial tissue damage in isolated rat hearts when delivered either before regional ischemia or at the time of reperfusion.

Cite This Study

Brar et al. (2000) studied this question.

synapsesocial.com/papers/6a96b831d45ba51c86b2e9ec https://doi.org/10.1074/jbc.275.12.8508

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Implication

Experimental study demonstrates that urocortin reduces cardiac damage in rat heart models, suggesting a therapeutic role during reperfusion after ischemic events.

Urocortin Protects against Ischemic and Reperfusion Injury via a MAPK-dependent Pathway

Discussion

Member takes

Implication

Key Points

Cite This Study

Urocortin Protects against Ischemic and Reperfusion Injury via a MAPK-dependent Pathway

Discussion

Member takes

Implication

Key Points

Cite This Study

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