What question did this study set out to answer?

To determine and compare the nucleotide sequences of the hemolysin structural gene across hemolytic and nonhemolytic biotypes of Vibrio cholerae.

June 1, 1988Infection and Immunity

Nucleotide sequences and comparison of the hemolysin determinants of Vibrio cholerae El Tor RV79(Hly+) and RV79(Hly-) and classical 569B(Hly-)

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Authors

ARAnne E. RaderUniversity of Nebraska Medical Center JMJohn R. MurphyJohns Hopkins University

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Implication

Genetic sequence analysis reveals an 11-base-pair deletion truncating hemolysin in classical Vibrio cholerae, indicating the genetic basis for biotype-specific hemolytic variation.

Key Points

To determine and compare the nucleotide sequences of the hemolysin structural gene across hemolytic and nonhemolytic biotypes of Vibrio cholerae.
Sequenced the hlyA structural gene from Vibrio cholerae El Tor biotype strains RV79(Hly+) and RV79(Hly-).
Sequenced and analyzed the hly determinant, promoter region, and predicted signal sequence of the nonhemolytic classical strain 569B(Hly-).
Both El Tor strains RV79(Hly+) and RV79(Hly-) share an identical 2,223-base open reading frame predicting an 81,977-dalton hemolysin precursor.
The classical 569B(Hly-) strain contains an 11-base-pair deletion within hlyA, predicting a truncated precursor of 26,765 daltons.
All examined strains possess identical regulatory regions for the putative hlyA promoter and the 25-amino-acid signal sequence.

Cite This Study

Rader et al. (1988) studied this question.

synapsesocial.com/papers/6a96ba6bdc0901934ff69a30 https://doi.org/10.1128/iai.56.6.1414-1419.1988

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Implication

Genetic sequence analysis reveals an 11-base-pair deletion truncating hemolysin in classical Vibrio cholerae, indicating the genetic basis for biotype-specific hemolytic variation.

Nucleotide sequences and comparison of the hemolysin determinants of Vibrio cholerae El Tor RV79(Hly+) and RV79(Hly-) and classical 569B(Hly-)

Discussion

Member takes

Implication

Key Points

Cite This Study

Nucleotide sequences and comparison of the hemolysin determinants of Vibrio cholerae El Tor RV79(Hly+) and RV79(Hly-) and classical 569B(Hly-)

Discussion

Member takes

Implication

Key Points

Cite This Study

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