Mouse lymphoid cells producing lytic antibody against alloantigens have been detected in vitro with the help of a plaque assay using allogeneic tumor cells as indicator cells. In C3H mice injected with 30 × 106 DBA/2 tumor cells, alloantibody plaque forming cells appeared in the spleen by day 3 and increased in number until day 9.
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Nordin et al. (1971) studied this question.
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