Equilibrium Dialysis Studies of the Binding of Thyroxine by Human Serum Albumin*

In previous work from this laboratory (1) studies on equilibrium dialysis were carried out to determine the binding constants for the inter- action of human serum albumin with thyroxine and its analogs.The classical technique of equi- librium dialysis as described by Scatchard and others (2-6) was employed.At present it seems clear that thyroxine in hu- man serum migrates to at least three different sites during electrophoresis at pH 8.6 (7-10).The three sites most frequently reported have been the thyroxine-binding a-globulin (TBG), albumin,