A reversed‐phase HPLC method using a Spherisorb NH 2 column and 85:15 v/v acetonitrile/water as mobile phase was developed for determination of starch and soluble sugars (glucose, fructose and sucrose) in plants. When applied to pure starch samples, the analytical method was as accurate as the official AOAC method and had better precision (coefficient of variation 0.87% vs 1.27%). When applied to green bean ( Phaseolus vulgaris L.), recovery was 97.7% for starch, 98.0% for glucose and 98.1% for both fructose and sucrose.
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López‐Hernández et al. (1994) studied this question.
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