Alterations in the structure of the ribose moiety of ATP reduce its effectiveness as a substrate for the sarcoplasmic reticulum ATPase.

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Population

Isolated sarcoplasmic reticulum vesicles

Comparison

Ribose-modified ATP analogs vs Unmodified ATP

Design

Preclinical

Authors

Discussion

3'-modified ATP analogs impair SERCA activation; leaves open whether such modifications can be exploited in cardiac disease models.

Population

Isolated sarcoplasmic reticulum vesicles

Intervention

Ribose-modified ATP analogs (modified at 2'- and 3'-positions)

Comparator

Unmodified ATP

Outcome

Steady state hydrolytic ratesurrogate

The 3'-hydroxyl group of ATP is critical for substrate specificity and high catalytic activity of the sarcoplasmic reticulum calcium ATPase.

Anderson et al. (1983) studied this question.

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