Bioreactor for enzymic reaction of fat and fatty acid derivatives. Part II. Some characteristics of continuous glyceride synthesis by lipase in a microporous hydrophobic membrane bioreactor.

Continuous synthesis of oleic acid glycerides by seven Upases available commercially were compared at 40°C using the microporous hydrophobic membrane bioreactor we developed recently. The lipases produced by Rhizopus japonicus, Candida cylindracea, and Phycomyces nitens were quickly inactivated in 97% glycerol solution and yielded very low glyceride conversions. The other four lipases yielded glyceride synthesis activities in the order: Rhizopus delemer's<Pseudomonas fluorescens's< Mucor miehei's