Key result
Monoclonal anti-idiotypic antibodies generated against TBE virus-neutralizing antibody failed to induce antiviral antibodies or protective immunity in syngeneic Balb/c mice.
The generated anti-idiotypic antibodies against TBE virus neutralizing antibody did not carry an internal image of the virus antigen and failed to induce protective immunity in mice.
Anti-idiotypic mAbs fail to induce TBE immunity in mice; leaves open whether alternative idiotypic constructs merit testing.
Seven monoclonal anti-idiotypic antibodies (anti-ID MAbs, Ab2) were generated against virus-neutralizing and hemagglutination-inhibiting monoclonal antibody (Ab1) specific for tick-borne encephalitis (TBE) virus. Six of these anti-ID MAbs inhibited the binding of Ab1 to the virus antigen, thus classifying these anti-ID antibodies as Ab2 beta or AB2 gamma. Inhibition tests with heterologous anti-TBE sera revealed that these anti-ID MAbs were not recognized by anti-TBE antibodies and therefore they do not carry an internal image of TBE virus antigen. Hence, the anti-ID MAbs may be classified as Ab2 gamma type. None of the anti-ID MAbs induced production of antiviral antibodies nor protective immunity in syngeneic Balb/c mice. Using these anti-ID MAbs four nonoverlapping idiotopes were identified on Ab1 variable region.
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Kopecký et al. (1995) studied Tick-borne encephalitis (TBE) virus. Monoclonal anti-idiotypic antibodies (anti-ID MAbs) was evaluated on Induction of antiviral antibodies or protective immunity. Monoclonal anti-idiotypic antibodies generated against TBE virus-neutralizing antibody failed to induce antiviral antibodies or protective immunity in syngeneic Balb/c mice.
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