Key result
An ELISA based on the chimeric antigen C21ErnsE2 detected 100% of sera from CSFV-infected pigs, compared to 90% using single-fragment ELISAs, allowing detection as early as 7 days postinfection.
Absolute Event Rate: 100% vs 90%
A chimeric antigen-based ELISA improves serodiagnostic sensitivity for classical swine fever virus, allowing detection as early as 7 days postinfection.
The antigenic region (residues 109 to 160) of classical swine fever virus (CSFV) protein E(rns) and the N-terminal antigenic region (residues 1 to 136) of protein E2 were constructed in the form of a fused, chimeric protein, C21E(rns)E2, for use as an enzyme-linked immunosorbent assay (ELISA) antigen for the serodiagnosis of CSFV infection. Tested with 238 negative-field (CSFV-free) sera from Canadian sources, the specificity of the ELISA was determined to be 93.7%. All 20 sera from experimentally infected pigs representing a variety of animals, virus strains, and days postinfection (dpi; range, 7 to 210) were detected as positive (100%). In contrast, an ELISA based on an E(rns) fragment (E(rns)(aa 109-160)) or an E2 fragment (E2(aa 1-221)) identified only 18 (90%) of 20 sera from infected pigs as positive, missing two targets collected at 7 dpi. These data suggest that use of the chimeric antigen C21E(rns)E2 would improve serodiagnostic sensitivity and allow for the detection of CSFV infection as early as 7 dpi.
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Lin et al. (2005) studied Classical swine fever virus (CSFV) infection (n=258). Chimeric protein C21ErnsE2-based ELISA vs. ELISA based on Erns fragment or E2 fragment was evaluated on Detection of positive sera from infected pigs (sensitivity). An ELISA based on the chimeric antigen C21ErnsE2 detected 100% of sera from CSFV-infected pigs, compared to 90% using single-fragment ELISAs, allowing detection as early as 7 days postinfection.
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