Key result
The Indian classical swine fever challenge virus is highly virulent, producing acute disease in piglets, and belongs to subgroup 1.2 under Group 1 based on phylogenetic analysis.
The Indian CSF challenge virus used for vaccine potency testing is a highly virulent strain belonging to subgroup 1.2 under Group 1.
Validates this strain for CSF vaccine potency testing in India; leaves open cross-protection against other subgroups.
AIM: The aim of this study was biological and molecular characterization of classical swine fever (CSF) challenge virus from India. MATERIALS AND METHODS: CSF challenge virus maintained at Division of Biological standardization was experimentally infected to two seronegative piglets. The biological characterization was done by clinical sign and symptoms along with postmortem findings. For molecular characterization 5'-nontranslated region, E2 and NS5B regions were amplified by reverse transcription polymerase chain reaction and sequenced. The sequences were compared with that of reference strains and the local field isolates to establish a phylogenetic relation. RESULTS: The virus produced symptoms of acute disease in the piglets with typical post-mortem lesions. Phylogenetic analysis of the three regions showed that the current Indian CSF Challenge virus is having maximum similarity with the BresciaX strain (USA) and Madhya Pradesh isolate (India) and is belonging to subgroup 1.2 under Group 1. CONCLUSION: Based on biological and molecular characterization of CSF challenge virus from India is described as a highly virulent virus belonging to subgroup 1.2 under Group 1 along with some field isolates from India and Brescia strain.
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Kumar et al. (2015) studied Classical swine fever (n=2). Classical swine fever challenge virus was evaluated on Biological and molecular characterization (clinical signs, postmortem lesions, and phylogenetic grouping). The Indian classical swine fever challenge virus is highly virulent, producing acute disease in piglets, and belongs to subgroup 1.2 under Group 1 based on phylogenetic analysis.
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