Separation of Mono-, Di-, and Triphosphate Nucleotides by Cytosine Substituted, Silica-Bound Sapphyrin Solid Supports

A cytosine-substituted sapphyrin when used as a silica gel bound HPLC solid support effectively separates guanosine 5′-mono-, di-, and triphosphate from a mixture of the mono-, di- and triphosphates of cytidine, uridine, adenosine, and guanosine, respectively under isocratic conditions at pH 7. In addition, using this same solid support, cytidine 5′-monophosphate, guanosine 5′-monophosphate, adenosine 5′-mono-phosphate and uridine 5′-monophosphate are all readily separated from each other under similar HPLC conditions.