Key result
A novel control using simultaneous diffusion of calcium and phosphate produced precipitate patterns indistinguishable from ATP incubation, demonstrating artifactual localization in high protein regions.
Population
single, unfixed, glycerol-extracted fibers from the pectoralis major muscle of leghorn chicks
Comparison
calcium precipitation method for localizing… vs novel control and classical controls
Design
Preclinical
Authors
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Questions ATPase localization validity in prior muscle studies; leaves open the need for validated controls in cytochemical assays.
The study demonstrates that the calcium precipitation method for localizing ATPase in muscle fibers can produce artifactual patterns due to high protein concentration, highlighting the need for novel controls in cytochemical procedures.
Bernard D. Tunik (1971) studied this question. Calcium precipitation method vs. Usual classical controls was evaluated on Localization of myofibrillar adenosine triphosphatase (ATPase) activity. A novel control using simultaneous diffusion of calcium and phosphate produced precipitate patterns indistinguishable from ATP incubation, demonstrating artifactual localization in high protein regions.
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