February 1, 1987Journal of Cellular Physiology
Phorbol esters induce angiogenesis in vitro from large‐vessel endothelial cells
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Discussion
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Implication
In vitro study demonstrates capillary-like sprouting in large-vessel endothelial cells exposed to phorbol esters, indicating macrovascular cells retain invasive angiogenic potential.
Key Points
- Determine whether the capacity to invade extracellular matrix and form capillary-like tubular structures in response to phorbol myristate acetate is unique to microvascular endothelial cells or also shared by large-vessel endothelium.
- Cultured endothelial cells isolated from human umbilical veins and calf pulmonary arteries on the surface of three-dimensional collagen gels.
- Treated the macrovascular cell cultures with the tumor promoter phorbol myristate acetate (PMA) to assess in vitro invasive tubulogenesis.
- PMA treatment stimulated both human umbilical vein and calf pulmonary artery endothelial cells to penetrate the underlying three-dimensional collagen matrix.
- Invading macrovascular cells formed vessel-like tubular structures within the collagen gels, demonstrating that large-vessel endothelium can express invasive angiogenic phenotypes.
Cite This Study
Montesano et al. (1987) studied this question.