Zonae pellucidae were isolated from oocytes obtained from rabbit and porcine ovaries. Antisera were prepared in rabbits against heat-solubilized porcine zonae and in guinea pigs against intact and heat-solubilized rabbit zonae and against purified rabbit zona proteins isolated from two-dimensional electrophoresis gels. These antisera were used in radioimmunoassay studies to show cross-reactivity between porcine and rabbit zona antigens. Immunoelectrophoresis studies also demonstrated the immunochemical cross-reactivity between two distinct antigens of rabbit and porcine zonae. These two rabbit zona antigens were demonstrated to be immunochemically identical to porcine zona antigens by crossed-line immunoelectrophoresis.
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Wood et al. (1981) studied this question.
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