Disinfection of explants for saffron (Crocus sativus) tissue culture

The choice of explant and its preparation are most likely one of the most important biotic factors that determine the success of a tissue culture protocol when the explant is derived from ex vitro organs. This review assesses the disinfection protocols available for saffron (Crocus sativus L.; Iridaceae) explants in an attempt to ascertain the most suitable set of parameters that could ensure successful tissue culture in subsequent treatments. From a methodological perspective, two explant types are most commonly adopted in saffron biotechnology, namely the use of fresh field-grown shoots, or dormant corms. The latter is more recommended to minimize contamination and to allow the use of more aggressive disinfection treatments.