Key result
RISC-sequencing of mouse cardiac transcriptomes identified 1645 mRNAs consistently targeted to cardiac RISCs, including 209 in vivo targets of miR-133a and 81 of miR-499.
Population
Mouse hearts (including those overexpressing miRs from Myh6 promoter-driven precursors)
Design
Preclinical
Authors
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RISC-seq enables sensitive cardiac miRNA target mapping in mice; leaves open human mechanistic insights and clinical translation.
RISC-sequencing provides a highly sensitive method for identifying in vivo microRNA targets in cardiac tissue, enabling better understanding of molecular mechanisms in cardiovascular disease.
Matkovich et al. (2010) studied this question. RISC-Seq vs. Transcriptome from the same individual hearts was evaluated on Identification of in vivo miR targets. RISC-sequencing of mouse cardiac transcriptomes identified 1645 mRNAs consistently targeted to cardiac RISCs, including 209 in vivo targets of miR-133a and 81 of miR-499.
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