Key result
TMB-8 (>0.5 mM) blocks secretion and induces leakage, while 0.15 mM enhances slow serotonin release but inhibits low affinity platelet factor 4 antigen release in washed platelets.
TMB-8 induces leakage of cytoplasmic content and specific loss of serotonin in washed platelets, which must be considered when using it as a calcium antagonist in experimental models.
Caution advised using TMB-8 in platelet experiments due to non-specific effects; leaves open its reliability as selective calcium antagonist.
8-(N,N-diethylamino)octyl 3,4,5-trimethoxybenzoate HCl (TMB-8) has been used to indicate involvement of Ca++ in platelet activities, but possible other effects have not been investigated. This study investigates TMB-8-induced leakage of cytoplasmic content and specific loss of one granule constituent, serotonin from washed platelets. TMB-8 concentrations above 0.5 mM block secretion initially and induce leakage after short time incubation. TMB-8 (0.15 mM) enhances a slow release of serotonin, but inhibits a slow release of low affinity platelet factor 4 antigen which take place when washed platelets are incubated at pH 7.4 and 37 degree C. These effects should be taken into consideration when TMB-8 is used as a calcium antagonist in platelets.
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Murer et al. (1982) studied Washed blood platelets. 8-(N,N-diethylamino)octyl 3,4,5-trimethoxybenzoate HCl (TMB-8) was evaluated on Leakage of cytoplasmic content and specific loss of serotonin. TMB-8 (>0.5 mM) blocks secretion and induces leakage, while 0.15 mM enhances slow serotonin release but inhibits low affinity platelet factor 4 antigen release in washed platelets.
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