The Morphology and Method of Division ofTrichomonas

Three trichomonads have been cultivated and studied,T. hominis, Trichomonassp. fromM. nemestrinus(= R.T. strain), andT. batrachorumfrom English frogs and toads. 2. A number of strains ofT. batrachorumwere cultivated over periods varying from two weeks to five months without the appearance of aEutrichomastixstage. 3. The division process ofT. batrachorumhas been studied. The new basal fibre arises as an outgrowth from the blepharoplast. The old axostyle is absorbed and new ones are formed from the daughter blepharoplast during the telophase. Six chromosomes appear during the prophase. Spindle fibres do not appear. There is a separation of the daughter blepharoplasts into centro-some and basal granule at the metaphase. 4. Division ofT. hominisandTrichomonassp. fromM. nemestrinusis essentially similar to that ofT. batrachorum. The number of chromosomes appeared to be five or six. Double individuals were found in the cultures, and inT. hominisindividuals containing four or six sets of organellae. Their significance is obscure.