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The use of a wax layer for root penetration tests under controlled soil conditions has made it possible to separate from other factors in the soil the effect of mechanical impedance on the growth of plant roots. The penetration percentage of cotton roots increased as soil moisture tension was increased from 0.05 bar to 0.52 bar then decreased as tension was further increased to 10.8 bars. The penetration also decreased with increasing salt content in the soil solution. Decreased aeration as measured by CO 2 content also lowered the penetrating ability of cotton roots. An increase in bulk density of the soil from 1.0 g. per cc. to 1.4 g. per cc. did not affect the penetrating ability of cotton roots into the wax layer.
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Gardner et al. (1964) studied this question.
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