What question did this study set out to answer?

To determine how HIV envelope glycoprotein gp120 affects the capacity of CD4+ T-helper cells to induce normal B-cell proliferation and antibody differentiation.

March 1, 1992BloodOpen Access

Inhibition of normal B-cell function by human immunodeficiency virus envelope glycoprotein, gp120

View Full Paper

Ask AI

Bookmark

Authors

NCNarendra ChirmuleSymphony Technology (United States)NONaoki OyaizuTokyo Medical and Dental University VKVS KalyanaramanCornell University

Discussion

Member takes

Implication

In vitro study demonstrates that HIV gp120 impairs CD4+ T-cell helper activity for B cells via direct contact interference, suggesting a mechanism for humoral immune dysfunction in HIV infection.

Key Points

To determine how HIV envelope glycoprotein gp120 affects the capacity of CD4+ T-helper cells to induce normal B-cell proliferation and antibody differentiation.
Evaluated antigen-induced proliferation and IgG secretion in autologous B cells co-cultured with CD4+ T cells, assessing physical contact requirements using Transwell membrane separation.
Pretreated CD4+ antigen-specific T-cell clones with purified gp120 in the presence or absence of soluble CD4, followed by functional activation assays.
Quantified T-cell secretion of interleukin-2 (IL-2), interleukin-4 (IL-4), and interleukin-6 (IL-6), and supplemented cultures with exogenous cytokines to test for functional rescue.
Pretreatment of CD4+ T cells with gp120 markedly suppressed autologous B-cell proliferation, polyclonal IgG secretion, and antigen-specific IgG production, which was specifically reversed by soluble CD4.
Functional impairment was observed only in T-cell-dependent fractionated small B cells and stemmed from disrupted T-B-cell contact rather than cytokine deficits alone.
Antigen-induced IL-2 and IL-4 release from T-cell clones was significantly reduced after gp120 exposure with no effect on IL-6, and adding exogenous cytokines failed to restore B-cell helper activity.

Cite This Study

Chirmule et al. (1992) studied this question.

synapsesocial.com/papers/6a97af68618eaafe842d2c99 https://doi.org/10.1182/blood.v79.5.1245.1245

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates that HIV gp120 impairs CD4+ T-cell helper activity for B cells via direct contact interference, suggesting a mechanism for humoral immune dysfunction in HIV infection.

Inhibition of normal B-cell function by human immunodeficiency virus envelope glycoprotein, gp120

Discussion

Member takes

Implication

Key Points

Cite This Study

Inhibition of normal B-cell function by human immunodeficiency virus envelope glycoprotein, gp120

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider