Preparation and Application of Core-Shell PMMA/Chitosan Nanoparticle

Objective To separate and enrich compounds from Apocynum venetum L. leaves. Methods The core-shell nanoparticles consisting of poly (methyl methacrylate) (PMMA) cores surrounded by various chitosan shells, induced by a tert-butylhydroperoxide (TBHP) solution. And core-shell nanoparticles was applied to immobilize Alpha glycosidase enzymes. Some compound from the extrat of Apocynum venetum L. leaves were absorbed by the immobilized Alpha glycosidase enzymes. and the components absorbed were detected by HPLC. Results Hyperoside and trifolin can be adsorbed by the nanoparticles immobilized Alpha glycosidase enzymes. Conclusion The method applied to separate and preconcentrate Apocynum leaves’ active ingredients is feasible.