Key result
Mutations in the untranslated hairpin of Turnip crinkle virus increased the mutation frequency up to 12-fold without inducing error catastrophe.
The hairpin repressor in Turnip crinkle virus may function as an RNA chaperone to properly fold the RNA-dependent RNA polymerase.
Hypothesis-generating for RNA hairpin regulation of viral fidelity; leaves open relevance to human or animal pathogens.
The mutation frequency of Turnip crinkle virus can increase 12-fold without inducing error catastrophe. Lesions in a hairpin repressor frequently reverted and led to second-site alterations biased for specific mutations. These results suggest that the hairpin may also function as an RNA chaperone to properly fold the RNA-dependent RNA polymerase.
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McCormack et al. (2004) studied Turnip crinkle virus (TCV) infection. Mutations in the H5 hairpin large symmetrical internal loop (LSL) vs. Wild-type TCV was evaluated on Mutation frequency (12-fold increase). Mutations in the untranslated hairpin of Turnip crinkle virus increased the mutation frequency up to 12-fold without inducing error catastrophe.
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