Experimental study reveals that polyphenol oxidase silencing suppresses leaf extract browning and protein crosslinking in Nicotiana benthamiana, highlighting utility for molecular pharming.
Oxidation in extracts from agroinfiltrated leaves leads to browning and protein precipitation. Here we show that silencing of polyphenol oxidase (PPO) in Nicotiana benthamiana suppresses enzymatic browning and avoids crosslinking of endogenous proteins such as RuBisCo. PPO silencing does not impact plant growth or development and may also enhance transient gene expression. These results show that depletion of PPO activity could be highly beneficial for plant science applications and molecular pharming.
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Mahadevan et al. (2025) studied this question.
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