Key result
Increasing loading stimulation frequency from 0.2 to 0.8 Hz enhanced the storage of [3H]acetylcholine from 19.5% to 34% of total tritium content in rat urinary bladder strips.
In rat urinary bladder strips, a loading frequency of 0.8 Hz optimally increases the formation and subsequent release of [3H]ACh during electrical stimulation.
May guide ex vivo bladder cholinergic models; leaves open translation to in vivo or human detrusor function.
The relationship between different frequencies of loading stimulation and [3H]acetylcholine (ACh) formation and release from nerve terminals has been investigated in extratrigonal strips of the urinary bladder of the rat. An increase in frequency (0.2, 0.4 and 0.8 Hz) for the 30 min incubations with [3H]choline produced an enhancement of storage of [3H]ACh from 19.5 to 34% of total tritium content in the tissue. Higher frequencies (1.6 and 3.2 Hz) failed to increase storage further on. The [3H]choline content did not vary significantly. Electrical field stimulation at 2 Hz (360 shocks) produced a release of tritium. The evoked outflow was higher when the strip was loaded at 0.8 Hz than at the other frequencies tested. Both [3H]ACh and [3H]choline were measured in the perfusate of strips preloaded at 0.8 Hz. Most of the induced outflow was found to be [3H]ACh, as in previous experiments carried out using 0.2 Hz as a loading frequency. The findings suggest that in the rat urinary bladder strip loading at 0.8 Hz is suitable for increasing the formation and the resulting release of [3H]ACh during electrical stimulation.
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D’Agostino et al. (1988) studied this question. Loading stimulation at different frequencies was evaluated on [3H]acetylcholine (ACh) formation and release. Increasing loading stimulation frequency from 0.2 to 0.8 Hz enhanced the storage of [3H]acetylcholine from 19.5% to 34% of total tritium content in rat urinary bladder strips.
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