Growth of protozoa in tissue culture. II.—Plasmodium relictum, exoerythrocytic forms

The exoerythrocytic forms of Plasmodium relictum have been grown in tissue culture, using the technique previously described for P. gallinaceum. Growth was terminated after 19 days. Fluid removed from the culture flasks after 5 and 9 days was infective for a canary.Growth took place in the presence of penicillin and of streptomycin.This technique is advantageous for studying the morphology of the exoerythrocytic forms, especially in the different phases of schizogony. The various stages are illustrated.