Selected strains of Trichoderma spp. were grown on 10 different solid media substrates and analyzed for the production of 6- n -pentyl-2 H -pyran-2-one (6PAP). Using the highest yielding medium from this study, ground corn plus a liquid supplement, 15 Trichoderma isolates were screened, and this led to the identification of two high yielding strains. By selection of solid media, liquid supplement, and harvest time, the yield of 6PAP was improved from ca. 200 up to about 1000 mg/kg. These results will provide an indication for future production studies leading to the development of a bulk-scale fermentation process.
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Cooney et al. (1997) studied this question.
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