Key result
Turkey reovirus S3 sequences from PES-affected flocks showed more amino acid substitutions compared to those from healthy flocks, clustering in clade III with 88.9-100% nucleotide identity.
Observational (n=29)
Turkey reovirus S3 gene sequences from PES-affected and healthy flocks cluster in clade III and show specific amino acid substitutions that may relate to virulence.
S3 substitutions may mark PES virulence in turkeys; hypothesis-generating for reovirus surveillance and requires experimental validation.
The molecular diversity in S3 gene sequences of turkey reovirus (TRV) was determined in poult enteritis syndrome (PES)-affected and apparently healthy turkey poults. Twenty-nine TRV-positive samples (15 from PES-affected flocks and 14 from apparently healthy flocks) were tested using self-designed primers for the S3 gene. Phylogenetic analysis revealed that the TRV S3 sequences of this study clustered in clade III and formed two different groups in this clade. The avian reoviruses from duck and goose formed clade I and those from chickens formed clade II. The clade III TRV sequences had a nucleotide percent identity of 88.9 to 100% among themselves but only of 59.5 to 63.5% and 69.2 to 72.6% with clades I and II, respectively. More amino acid substitutions were present in TRVs from PES-affected flocks than in those from apparently healthy flocks using ATCC VR-818 (AY444912) as a benchmark. All TRVs of this study showed substitutions at positions 244 and 285. The impact of these changes on the virulence of the virus, if any, needs to be studied.
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Jindal et al. (2014) conducted an observational in Poult enteritis syndrome (PES) (n=29). PES-affected flocks vs. Apparently healthy flocks was evaluated on Molecular diversity and phylogenetic clustering of S3 gene sequences. Turkey reovirus S3 sequences from PES-affected flocks showed more amino acid substitutions compared to those from healthy flocks, clustering in clade III with 88.9-100% nucleotide identity.
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