ORK1, a potassium-selective leak channel with two pore domains cloned from Drosophila melanogaster by expression in Saccharomyces cerevisiae
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Key result
Expression of the ORK1 gene from Drosophila melanogaster yielded an ungated K+-selective leak current in Xenopus oocytes and conferred K+ transport capacity in yeast.
Population
Drosophila melanogaster, Saccharomyces cerevisiae strain CY162, and Xenopus laevis oocytes
Comparison
Heterologous expression of the ORK1 potassium… vs Control yeast strains and control oocytes
Design
Preclinical
Authors
Discussion
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Implication
Provides molecular candidate for resting K+ leak in excitable tissues; extends two-pore domain channel family but leaves open mammalian and clinical relevance.
Structured PICO
P
Population
Drosophila melanogaster (third-instar larval mRNA, adult flies), Saccharomyces cerevisiae strain CY162 (defective for K+ uptake), and Xenopus laevis oocytes
I
Intervention
Heterologous expression of the ORK1 potassium channel gene
C
Comparator
Control yeast strains (vector alone or expressing KAT1) and control oocytes (water injected)
O
Outcome
K+ transport capacity (yeast growth on low K+ medium, 86Rb+ uptake) and electrophysiological properties of K+-selective currents (in oocytes)surrogate
The cloning and characterization of ORK1 identifies a novel two-pore domain potassium channel that functions as an open rectifier, providing a molecular candidate for the leak conductances that mediate resting membrane potential in excitable tissues.
Cite This Study
Goldstein et al. (1996) studied this question. ORK1 gene expression was evaluated on K+ transport capacity and K+-selective current. Expression of the ORK1 gene from Drosophila melanogaster yielded an ungated K+-selective leak current in Xenopus oocytes and conferred K+ transport capacity in yeast.
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