Mutanase (Mutanase Novo) affects the high frequency production of protoplasts in the following strains of yeast: Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Trichosporon pullulans, and Schwanniomyces alluvius. Regeneration frequencies varied with the strain used and ranged between 10 and 18%. This enzyme preparation appears to be a very useful means of obtaining protoplasts from a wide variety of yeasts currently being used for experimental purposes.
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Stephen et al. (1981) studied this question.
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