What question did this study set out to answer?

To evaluate BODIPY TR methyl ester dye as a vital counterstain spectrally distinct from GFP for live dual-channel imaging of embryonic morphogenesis.

December 21, 2004Developmental DynamicsOpen Access

Visualizing morphogenesis in transgenic zebrafish embryos using BODIPY TR methyl ester dye as a vital counterstain for GFP

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To evaluate BODIPY TR methyl ester dye as a vital counterstain spectrally distinct from GFP for live dual-channel imaging of embryonic morphogenesis.
Applied BODIPY TR methyl ester dye to living transgenic zebrafish embryos expressing green fluorescent protein (GFP).
Performed dual-channel 3D and 4D live confocal microscopy paired with computer-assisted rendering to capture tissue dynamics.
BODIPY TR methyl ester exhibited clean spectral separation from GFP without emission overlap, enabling simultaneous dual-channel visualization of target cells and whole-embryo anatomical context.
The photostable dye successfully supported the acquisition and reconstruction of high-resolution 3D and 4D datasets tracking cell behavior during morphogenesis.

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Implication

Experimental imaging reveals BODIPY TR methyl ester serves as an effective vital counterstain alongside GFP in transgenic zebrafish embryos, facilitating high-resolution 3D and 4D morphogenesis...

Cite This Study

Cooper et al. (2004) studied this question.

synapsesocial.com/papers/6a97dcdca9054fc672c6140a https://doi.org/10.1002/dvdy.20252

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Implication

Experimental imaging reveals BODIPY TR methyl ester serves as an effective vital counterstain alongside GFP in transgenic zebrafish embryos, facilitating high-resolution 3D and 4D morphogenesis...

Visualizing morphogenesis in transgenic zebrafish embryos using BODIPY TR methyl ester dye as a vital counterstain for GFP

Key Points

Discussion

Implication

Cite This Study

Visualizing morphogenesis in transgenic zebrafish embryos using BODIPY TR methyl ester dye as a vital counterstain for GFP

Key Points

Discussion

Implication

Cite This Study

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