What question did this study set out to answer?

To determine whether mRNA profiling of urinary extracellular vesicles can uncover pathogenic mechanisms and serve as non-invasive biomarkers for diabetic kidney disease.

April 19, 2023iScienceOpen Access

Genome-wide mRNA profiling in urinary extracellular vesicles reveals stress gene signature for diabetic kidney disease

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Authors

ODOm Prakash DwivediInstitute for Molecular Medicine Finland KBKarina BarreiroUniversity of Helsinki AKAnnemari KäräjämäkiiRobot (United States)

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Implication

Transcriptomic study reveals an extracellular vesicle stress gene signature in diabetic kidney disease, indicating potential for early non-invasive detection.

Key Points

To determine whether mRNA profiling of urinary extracellular vesicles can uncover pathogenic mechanisms and serve as non-invasive biomarkers for diabetic kidney disease.
Conducted genome-wide sequencing of approximately 200 urinary extracellular vesicle mRNA samples from clinical cohorts of individuals with type 1 diabetes, with replication in type 1 and type 2 diabetes.
Identified disease-associated transcripts and built a predictive multigene transcriptional stress score evaluated against longitudinal kidney function decline.
Sequencing consistently detected over 10,000 mRNAs mirroring the kidney transcriptome, revealing 13 proximal tubule genes linked to hyperglycemia and cellular oxidative stress that were upregulated in diabetic kidney disease.
A transcriptional stress score composed of six genes (GPX3, NOX4, MSRB, MSRA, HRSP12, and CRYAB) tracked long-term renal function loss and detected early decline in normoalbuminuric individuals.

Cite This Study

Dwivedi et al. (2023) studied this question.

synapsesocial.com/papers/6a97deec894a49665d15d9d8 https://doi.org/10.1016/j.isci.2023.106686

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Implication

Transcriptomic study reveals an extracellular vesicle stress gene signature in diabetic kidney disease, indicating potential for early non-invasive detection.

Genome-wide mRNA profiling in urinary extracellular vesicles reveals stress gene signature for diabetic kidney disease

Discussion

Member takes

Implication

Key Points

Cite This Study

Genome-wide mRNA profiling in urinary extracellular vesicles reveals stress gene signature for diabetic kidney disease

Discussion

Member takes

Implication

Key Points

Cite This Study

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