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January 1, 1981Nucleic Acids ResearchOpen Access

Requirement for the C-terminal region of middle T-antigen in cellular transformation by virus

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Key result

Removal of the termination codon or any amino acid coding sequences of the viral middle T-antigen caused a drastic decrease in the transforming ability of the DNA in Rat-1 cells.

Population

Rat-1 cells in culture

Comparison

Transfection with plasmids containing viral DNA… vs Transfection with plasmids containing viral DNA…

Design

Preclinical

Authors

UNUlrike NovakThe Honourable Society of Lincoln's InnBGBeverly E. GriffinImperial College London

Discussion

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Implication

Essential C-terminus of middle T-antigen required for transformation; leaves open its relevance to human polyomavirus oncogenesis.

Structured PICO

P
Population
Rat-1 cells in culture
I
Intervention
Transfection with plasmids containing viral DNA with deletions in the C-terminal region of middle T-antigen (including the termination codon or amino acid coding sequences)
C
Comparator
Transfection with plasmids containing viral DNA with deletions between the presumed translational termination codon and the single viral EcoR I site
O
Outcome
Cellular transformationsurrogate

The C-terminal region of the polyoma virus middle T-antigen is essential for its ability to induce cellular transformation.

Cite This Study

Novak et al. (1981) studied Cellular transformation by polyoma virus. Deletions of polyoma virus DNA around the C-terminus of middle T-antigen vs. Intact viral DNA / DNA without deletion of termination codon or amino acid coding sequences was evaluated on Ability to transform Rat-1 cells in culture. Removal of the termination codon or any amino acid coding sequences of the viral middle T-antigen caused a drastic decrease in the transforming ability of the DNA in Rat-1 cells.

synapsesocial.com/papers/6a97e14dcfa25b768ec62c74https://doi.org/10.1093/nar/9.9.2055
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