Fabrication, In Vitro and In Vivo Evaluation of Bioactive Glass‐Doped Biodegradable Bilayer Polymeric Dental Nanofibrous Membranes for Guided Tissue Regeneration Applications
Preclinical study demonstrates enhanced cell proliferation and tissue integration with bioactive glass bilayer membranes, highlighting potential for guided bone regeneration.
Key Points
To fabricate and evaluate a biodegradable, bioactive glass-doped bilayer nanofibrous membrane engineered to support both soft and hard tissue regeneration in dental guided bone regeneration applications.
Fabricated bilayer membranes via electrospinning, creating a soft-tissue interface layer from polyvinyl alcohol (PVA) with 3% 45S5 bioactive glass (BG) and a hard-tissue interface layer from PLGA/PHBV blend polymers with 10% BG.
Characterized mechanical, thermal, physicochemical, and bioresorption properties, followed by in vitro and in vivo animal assays assessing fibroblast and bone cell biocompatibility, proliferation, and adhesion.
Bilayer nanofiber membranes exhibited suitable mechanical stability, thermal properties, bioactivity, and bioresorbability tailored for guided tissue regeneration.
In vitro and in vivo assays revealed high biocompatibility with fibroblast and bone cells, showing accelerated cell proliferation and enhanced adhesion to the membrane surface.