Controlled animal study demonstrates gut microbiome remodeling without adverse metabolic shifts in mice, suggesting probiotic potential for Enterococcus sp. SB12.
Key Points
To evaluate the nutritional profile of Enterococcus sp. SB12 and determine its impact on gut microbiota, blood biochemistry, and oxidative stress markers in mice.
Quantified vitamin and amino acid profiles in Enterococcus sp. SB12 biomass using high-performance liquid chromatography (HPLC).
Administered Enterococcus sp. SB12 daily via drinking water at 1 × 10^8 CFU/g body weight for 29 days to female mice divided into experimental and control groups (N=20; n=10 per group).
Characterized fecal microbiota using 16S rRNA gene and ITS2 sequencing and measured blood biochemical markers, lipid panels, and oxidative stress indicators.
HPLC detected vitamins B1, B3, B5, B6, and C, as well as key amino acids such as valine (9.27 mg/g), cysteine (5.80 mg/g), and glutamine (5.34 mg/g) in the bacterial biomass.
Treated mice displayed increased relative abundance of Enterococcus and Firmicutes-associated taxa alongside reduced abundance of Desulfovibrionaceae and Helicobacter-associated taxa.
Mice receiving SB12 exhibited significantly higher body weight (28.1 ± 1.05 g vs. 27.2 ± 0.82 g; p < 0.05), while visceral fat mass, organ weights, lipid profiles, blood glucose, and oxidative stress markers showed no significant differences.