April 1, 1980Journal of General Virology
Monoclonal Antibodies to Influenza Matrix Protein: Detection of Low Levels of Matrix Protein on Abortively Infected Cells
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Discussion
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Implication
In vitro assay demonstrates low influenza matrix protein surface levels on infected cells, suggesting it is unlikely the primary target for cross-reactive cytotoxic T cells.
Key Points
- To quantify the surface expression of type A influenza virus matrix (M-) protein on infected cells and evaluate its plausibility as the target for cross-reactive cytotoxic T-lymphocytes.
- Quantified surface M-protein expression on type A influenza virus-infected P815 mastocytoma cells up to 16 hours post-infection using an M-protein-specific monoclonal antibody.
- Only low levels of approximately 10^3 M-protein molecules per cell were detected on the cell surface up to 16 hours post-infection.
- At 3 to 5 hours post-infection, when P815 cells serve as effective targets for cross-reactive T-cell lysis, only approximately 10^2 M-protein sites per cell were present.
Cite This Study
Hackett et al. (1980) studied this question.