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November 15, 2007Journal of VirologyOpen Access

A Conserved Motif in Region V of the Large Polymerase Proteins of Nonsegmented Negative-Sense RNA Viruses That Is Essential for mRNA Capping

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Key result

A new motif, GxxT[n]HR, present within conserved region V of the L protein of nonsegmented negative-sense RNA viruses, is essential for their unconventional mechanism of mRNA cap formation.

Population

Vesicular stomatitis virus (prototype NNS RNA virus) L proteins

Design

Preclinical

Authors

JLJianrong LiBaylor College of MedicineARAmal RahmehUniversitat Pompeu FabraMMMarco J. MorelliVita-Salute San Raffaele University

Discussion

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Implication

May enable antiviral targeting of NNS RNA viruses; leaves open feasibility in mammalian models.

Key Points

  • Identify the amino acid residues in the large polymerase protein of nonsegmented negative-sense RNA viruses required for unconventional mRNA cap formation.
  • Reconstituted the in vitro mRNA capping reaction using highly purified components of the prototype vesicular stomatitis virus.
  • Engineered and screened a panel of viral large (L) proteins containing single-amino-acid substitutions at universally conserved positions.
  • Identified a novel, universally conserved motif designated GxxT[n]HR located within region V of the viral L protein.
  • Established that the GxxT[n]HR sequence motif is essential for polyribonucleotidyltransferase activity and mRNA cap formation in nonsegmented negative-sense RNA viruses.

Structured PICO

P
Population
Vesicular stomatitis virus (prototype NNS RNA virus) L proteins
I
Intervention
Single-amino-acid substitutions to universally conserved residues in L protein
O
Outcome
mRNA cap formation

Identifies a novel GxxT[n]HR motif in the L protein of NNS RNA viruses essential for their unique mRNA capping mechanism.

Cite This Study

Li et al. (2007) studied Nonsegmented negative-sense RNA virus infection. Single-amino-acid substitutions in L protein vs. Wild-type L protein was evaluated on mRNA cap formation. A new motif, GxxT[n]HR, present within conserved region V of the L protein of nonsegmented negative-sense RNA viruses, is essential for their unconventional mechanism of mRNA cap formation.

synapsesocial.com/papers/6a97efead08ffd74dfc637a3https://doi.org/10.1128/jvi.02107-07
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Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Amino Acid Residues within Conserved Domain VI of the Vesicular Stomatitis Virus Large Polymerase Protein Essential for mRNA Cap Methyltransferase Activity2005 · 119 citations
  2. 2RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination1977 · 3,485 citations