Key result
IgG autoantibodies against peptides bearing three post-translational modifications were significantly associated with severe joint damage and higher median titers in RA-ILD patients.
Why the study?
Proteins resulting from post-translational modifications can trigger autoimmune responses in rheumatoid arthritis, prompting investigation into anti-AMPA autoantibodies as biomarkers for severe disease and interstitial lung disease.
Are anti-AMPA autoantibodies against multi-modified peptides associated with severe rheumatoid arthritis phenotypes and interstitial lung disease?
Observational
Are anti-AMPA autoantibodies against multi-modified peptides associated with severe rheumatoid arthritis phenotypes and interstitial lung disease?
Autoantibodies against peptides with multiple post-translational modifications may serve as biomarkers for severe rheumatoid arthritis phenotypes, including erosive disease and interstitial lung disease.
Multi-PTM chimeric fibrin peptides may complement ACPA testing in some seronegative RA patients; leaves open diagnostic or prognostic utility.
Rheumatoid arthritis (RA) is characterized by the presence of autoantibodies that are of paramount importance for the diagnosis and prognosis of the disease and have been implicated in its pathogenesis. Proteins resulting from post-translational modifications (PTMs) are capable of triggering autoimmune responses important for the development of RA. In this work, we investigate serum antibody reactivity in patients with an established RA against a panel of chimeric peptides derived from fibrin and filaggrin proteins and bearing from one to three PTMs (citrullination, carbamylation and acetylation) by home-designed ELISA tests (anti-AMPA autoantibodies). The role of anti-AMPAs as biomarkers linked to the presence of a more severe RA phenotype (erosive disease with radiological structural damage) and to the presence of interstitial lung disease (ILD), a severe extra-articular manifestation in RA patients entailing a high mortality, was also analyzed. In general, the association with the clinical phenotype of RA was confirmed with the different autoantibodies, and especially for IgA and IgM isotypes. The prevalence of severe joint damage was only statistically significant for the IgG isotype when working with the peptide bearing three PTMs. Furthermore, the median titers were significantly higher in patients with RA-ILD, a finding not observed for the IgG isotype when working with the single- and double-modified peptides.
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García-Moreno et al. (2021) conducted an observational in Rheumatoid arthritis. Anti-AMPA autoantibodies was evaluated on Severe RA phenotype (erosive disease with radiological structural damage) and interstitial lung disease (ILD). IgG autoantibodies against peptides bearing three post-translational modifications were significantly associated with severe joint damage and higher median titers in RA-ILD patients.
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