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Graphite oxide (GO) was effectively prepared by Hummers and Offeman method and it possesses different types of oxygen functionalities which allows GO to interact with organic and inorganic materials in covalent, noncovalent or ionic manner. The GO was reduced by hydrazine hydrate and sodium borohydride as reducing agents to obtain graphene as end product. Characterization of both GO and G was carried out by SEM, TEM, UV-Vis spectroscopy and FTIR. Due to this the sp 3 hybridization of GO will convert to sp 2 hybridizaton by removing oxygen from GO. Graphene has various applications, however water soluble graphene could be better option for biomedical applications.
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Ankamwar et al. (2012) studied this question.
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