In vitro development of tooth rudiments of embryonic mice

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Embryonic mouse mandibular tooth rudiments, completely excised from the host and placed in vitro at a “glass‐plasma clot” interface, fail to develop normally. The organization of the rudiment is destroyed by migration of cells into the cloth. Rudiment morphology is maintained and histodifferentiation occurs when such rudiments are grown on artificial filter membranes. In cultures of 16 day embryonic incisors, a “refractile” material is noted on the distal‐incisal‐labial side of the developing rudiment after three days in vitro growth; this material increases in extent during subsequent growth. Histological sections show normal differentiation of the cells of the rudiment; extracellular matrices are deposited by the odontocytes and also by amelocytes. The position of amelocyte matrix correlates with that of the “refracticle” material seen in the living culture. The sequence of changes during cellular differentiation in vitro corresponds to that occurring in vivo.